Sentence examples for using the sanger methodology from inspiring English sources

The amplicons were sequenced using the Sanger methodology in a MicroAmp – Optical 96-well reaction plate (Applied Biosystems, USA) and the automatic sequencer ABI 3130 (Applied Biosystems, USA).

Since there was some discrepancies between the two technologies that utilized DNA obtained from the original frozen stock of the PB12 strain (see Materials and Methods), we decided to sequence each of the mutant genes solely reported by one company, after PCR amplification using the Sanger methodology.

Using the Sanger strategy, the genome sequence of PA64s now comprises 382 Mb.

The E. coli DH10B strain was used as the cloning host, and six clones were selected to be sequenced using the Sanger method at MACROGEN (Seoul, South Korea).

PCR products were sequenced using the Sanger method.

The p.Q485H mutation identified via WES was confirmed using the Sanger method.

Each sequence generated using the Sanger method had a perfect match to overlapping fragments.

Sequencing was done at the DOE Joint Genome Institute JGII) using the Sanger method.

> A short insert library was sequenced using the Sanger procedure.

DNA regions containing putative mutations in regulatory genes detected by RN and WG were PCR amplified using oligonucleotide primers listed in table S3 presented in Additional file 4, purified by the Pure Link PCR purification kit and sequenced by the Sanger methodology with the Taq FS Dye Terminator Cycle Sequencing Fluorescence-Based Sequencing, in a Perkin Elmer/Applied Biosystems Model 3730.

Therefore, it is likely that the mutations in the PB12 strain not confirmed by the Sanger methodology are false positives.