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Using the samples described above, we repeated nested PCRs five times on all 95 individuals, one PCR plate per day for 5 days using the same tick DNA extractions, PCR reagents, and thermocycler.
Informed consent from the patients and/or their care providers for using the samples described in this study was obtained by the Bryan Alzheimer's Disease Research Center BADRCC) of Duke University Medical Center, the Knight ADRC (KADRC) of Washington University School of Medicine in St . Louis(MO), and the Instituto Neurologico de Colombia (Colombian Neurological Institute, INDEC).
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The major limitation of using the sample described in this study is that younger people are under-represented.
(b) Immunoblot analyses of the samples described above using antibodies against specific subunits of Integrator and Mediator.
Briefly, cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, University Park, IL, USA) using the RNA samples described.
Briefly, cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., University Park, IL, USA) using the RNA samples described.
Using the sampling weights described below, this sample represents around 100 million people, 10 million of whom have COPD.
The depth distribution of the NCs was measured by transmission electron microscopy (XTEM) using the sample preparation method described in [7].
Samples from the posterior distribution, equation [6], were obtained using the Gibbs sampler described by de los Campos et al. (2010).
Model-based estimation using the sample design we describe provides a transparent method for estimating biomass for particular spatial domains.
Optimum results were obtained using the sample pretreatment extraction methods described below and was used for all subsequent analyses.
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