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The major limitation of using the sample described in this study is that younger people are under-represented.
Due to a low response rate using the sample described above (154/644; 23.9%), we expanded the survey population to recruit participants from the membership of the Maternal Infant Child and Youth Research Network (MICYRN), a Canadian network linking investigators from 17 academic health centres involved in pediatric clinical research.
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Using the samples described above, we repeated nested PCRs five times on all 95 individuals, one PCR plate per day for 5 days using the same tick DNA extractions, PCR reagents, and thermocycler.
We have applied the wavelet-based approach to analyze the geomagnetic time series observed in a sequence from time scales of years to months using the sampling intervals described above.
Using the sampling weights described below, this sample represents around 100 million people, 10 million of whom have COPD.
The depth distribution of the NCs was measured by transmission electron microscopy (XTEM) using the sample preparation method described in [7].
Model-based estimation using the sample design we describe provides a transparent method for estimating biomass for particular spatial domains.
Optimum results were obtained using the sample pretreatment extraction methods described below and was used for all subsequent analyses.
Demographic characteristics for the overall sample are described using the sample mean and standard deviation for continuous variables and the frequency and percentage for categorical variables.
Briefly, cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, University Park, IL, USA) using the RNA samples described.
Briefly, cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., University Park, IL, USA) using the RNA samples described.
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