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Extended x-ray absorption fine structure (EXAFS) data were transformed into k space (χ spectra) using the sample absorption edge.
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Maps were plotted using the absorption spectra (maximum absorption is represented in red, and minimum absorption is represented in blue).
Urine samples were analyzed directly using the atomic absorption spectrophotometer without acid treatment.
Dry samples were digested and analysed for Cu and Zn using the atomic absorption spectrophotometry.
Purity of each RNA sample was determined using the absorption ratio (260/280 nm) determined by NanoDrop 1000 (Pequlab, Erlangen, Germany).
The integrity of RNA samples was determined on agarose gels (1.2%) and spectrophotometrically, using the absorption ratio at 260/280 nm.
An additional disposable ultra-thin glass substrate (∼30 µm thick) was also used between the sample and the absorption filter.
RNA concentrations were determined by absorption at 260 nm using the Nanodrop 2000 (Thermo) and absorption ratios A260/A280 and A260/A230 were used to assess sample integrity and purity.
The solutions of the digested samples were determined using the inductively coupled plasma-atomic absorption spectrometry.
Absorption of samples was measured using the surface thermal lensing (STL) technique.
'Loss of viability, %' was calculated using the following equation: (U− S / U*100, where U is the absorption value of the untreated control and S is the absorption value of sample.
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