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The recombinant strain expressing immunotoxin (αCD22HCH23PE40) was previously engineered using the same vector (Figure 1a) (Tran et al. 2013b).
The upper graph displays the low-pass-filtered ASM scalar residuals obtained with the ASM-V product (daily vector calibration), while the lower ones display the ASM vector data using the same vector algorithm with a single and constant set of vector calibration parameters without (middle graph) and with (bottom graph) external inputs for the correction of seasonal effects.
However, using the same vector and repeat type [23], we did not observe toxicity upon induction of the (CUG 480 expansions in our transgenic lines.
In this study, reduced ZO-1 expression in RPE cells using the same vector also resulted in an increase in ZONAB staining.
To quantify VLPs, Reverse transcriptase activity was determined and compared to that of PBj vectors of known infectivity on HT1080 cells, assuming that RT activity is equal for infectious and non-infectious particles generated using the same vector genome.
Both luciferases were fused with the PEST element of mouse ornithine decarboxylase and were expressed in NIH3T3 cells under the control of the mouse clock gene (mPer2) promoter using the same vector backbone.
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The expression of the target proteins using the same vectors in vitro showed that differences in expression level were wholly dependent on the environment of the expressing cell and an investigation of the time course of expression showed it could affect substantially the observed expression level for poorly but not well-expressed proteins.
Stable transfection in HEK293/EBNA cells was achieved using the same vectors and Fugene 6 method as previously described.
P. yoelii GFP transgenic lines of 17XL and 17X were generated using the same vectors, targeting strategy and protocols described elsewhere for P. berghei (Franke-Fayard et al., 2004).
Studies were conducted using the same vectors as used in the cytotoxicity study comprising HeLa cells and MRC-5s: the trans-complemented LVVs INwt+IN-I-PpoIH78A and IND64V+IN-I-PpoIH78A. IND64V+IN-I-PpoIH78A. IND64V+IN-I-PpoIH78A.oSince DNA sequences of the latest human genome verDNAn GRCh37/Hg19, hiss inothe unplaced contincluded_gl000220 were counted and regarded as rDNA intheachromosomal].
As the control vector, we used the same vector containing a sequence targeting luciferase, TGACCAGGCATTCACAGAAAT.
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