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These experiments were also made in 15-L glass aquaria using the same treatments and experimental conditions as described above for the experiment on biochemical parameters.
No significant variations in mPRα gene and protein expression was observed using the same treatments (data not shown).
The intensity of DCF fluorescence thus correlated well with the observed cell death using the same treatments (cf. Fig. 7 and Fig. 8).
In addition, the inhibitory effect on the growth of gallbladder cancer xenograft on nude mice was evaluated using the same treatments as those described above.
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Blank samples were prepared in triplicate and treated in parallel with algae samples using the same treatment procedure.
We tried to improve the reliability of treatment implementation by using the same treatment and providing the same training, with the same instructor for all subjects of the experimental group.
Notably, these results were comparable to cy5**-cMBP fluorescence imaging using the same treatment models with an optimal dose of 1 nmol and optimal imaging time of 60 min p.i.., and this was validated by histologic and immunohistochemical analyses.
While previous studies have shown beneficial effects of vitamin C, such as reduced transcapillary leakage, reduced lymph flow and reduced oedema formation, using the same treatment regimes that were used in the present study [4, 6, 9, 10], we could not demonstrate any effects on the plasma volume loss.
A higher efficiency in surface hardening is shown by the glow-discharge treatment in respect of the furnace process: by using the same treatment temperature and time, glow-discharge treated samples have thicker hardened layers with higher hardness values than the furnace treated ones, and this effect is more marked for 973-K treated samples, while it is reduced for 1173-K treated samples.
The plasma treatment is shown to be more efficient in hardening titanium surface layers than the furnace process: by using the same treatment temperature and time, plasma treated samples have a thicker hardened layer, with higher hardness values than the furnace treated ones.
As the depth of the functionalized interfacial region increases, a longer time is needed for the polar side groups to reorient (the first step) and for unoxidized P4MS molecules to migrate to the surface (the second step), which resulted in the sample with deeper functionalized region having lower reconstruction rate and RD using the same treatment condition.
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