Sentence examples for using the same transfection from inspiring English sources

In the other set of cells, an equal amount of the mutant form of the above promoter (FOP flash) was co-transfected using the same transfection reagent.

Using the same transfection protocol outlined above, MiaPaCa2 parental cells were transfected with equal amounts of pp32-encoding and empty vector pcDNA in T-75 flasks.

For plasmid transfection, 1  μg of plasmid was transfected to each well of one six-well plate using the same transfection methods mentioned above.

Each virus was produced at the same time, using the same transfection protocol adjusting for second or third generation lentiviruses.

Using the same transfection method, we introduced inhibitors of these miRNAs into normoxic CNE cells, which express low levels of VEGF and high levels of endogenous miR-15b, miR-16, miR-20a, and miR-20b.

Lipofection systems were also assessed at 12°C and 37°C using the same transfection condition and HtTA HeLa cells.

We also examined the effect of a different FOXO factor, namely FOXO1a, on ER-α and ER-β activity using the same co-transfection approach and found similar results.

Forced induction of ODC activity in normal cells did not trigger oncogenic transformation [e.g., 47] although some authors [e.g., 48] reported opposite results using the same cells but different transfection vectors and ODC gene (murine versus human).

Initial reverse and 24 h later forward transfection procedures were conducted using the same amounts of siRNAs and transfection reagent.

The second round of transfection was performed 48 hours after the first transfection, using the same method.

To investigate the nature of these interactions we performed a series of EMSA experiments using the same mutations as in the transfection experiments and wt (wild type) sequences (Fig. 5D F).