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Also, other functional variants may affect the expression of PRNP and therefore carrying out confirmatory studies in these loci or other polymorphisms using the same tissue or other tissues in other populations would be useful.
The twins were autopsied at the same facility using the same tissue processing protocols.
Prior studies have focused on elucidating genes whose expression differs both between individuals and within the same individual, using the same tissue type.
Testing was done using the same tissue that had been used for gene expression arrays, and was performed for 31 genes.
Using the same tissue extract as described above, 20 µl aliquots were added in triplicate to wells of a 96 well UV transparent microtitre plate (Costar).
Infectious virus was quantified in tissues, using the plaque assay, and compared to the results obtained by quantitative PCR using the same tissue preparation and normalized to 1 mL tissue homogenate.
Similar(37)
The above agree with results from Daniels et al. [ 10] using the same tissues as in the present study.
To examine these protein expression levels, western blot analysis was also performed using the same tissues (Fig. 5).
Lymph nodal tissues were used as a positive control, while a negative control was achieved using the same tissues but omitting the primary antibody.
Quantitative RT-PCR was conducted for some genes using the same tissues tested (head, abdomen, thoracopods and photophores) to confirm the integrity and robustness of EST sequencing.
Using the same tissues, we quantified the relative abundance of two barley mRNAs, coding for an SPL gene predicted to be targeted by miR156, HvSPL (U21_18637) and a potential target of miR172, HvAP2L (U21_18652).
More suggestions(15)
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