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On receipt of the samples all swabs are immediately inoculated directly onto chromogenic media and then using the same swabs the DNA is extracted using the BD GeneOhm protocol.
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The abdomen (flank), thorax (lateral) and breast (lateral); which are sites with the highest rate of contamination (ISO 17604, [2005]); were chosen for sampling using the same swab over all the sites.
Using the same swab, the process was repeated in the other nostril.
In addition, a broth enrichment containing Mueller Hinton broth with 6.5% NaCl was inoculated using the same swab, and this was incubated overnight at 35°C.
We also sampled the wall or ceiling of hibernacula under, near (10 20 cm), and far from (>2 m) roosting bats by using the same swabbing technique.
All participating countries used the same swab technique, culture and typing methods, which were validated [ 33].
They were swabbed in the cloacae using the same type of Amies gel swabs as used for the geese.
DNA was extracted from acute-phase serum and the eschar swab by using the same procedure as had been used for the first patient.
The plates were incubated and screened, and suspect colonies were identified by using the same laboratory methods used for the swab and wipe samples.
Samples of lesions found in internal organs and tissues during necropsies were taken with sterile swabs and cultured using the same standard microbiology protocols.
Buccal mucous membrane and skin swabs are analyzed by using the same IS-pro technique.
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