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To estimate the efficiency of the method, we sequentially introduced a series of sequence insertions at four distinct sites into our model target DNA using the same suppression oligonucleotides SO1 and SO2 and internal mutagenic oligonucleotides listed in the Additional File 2. The mutation series included four 1 bp sequence insertions, four 50 bp insertions and four 100 bp insertions.
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Notably, a recent study demonstrated reduced stem cell activity in response to Notch1 or Notch4 suppression using the same breast cancer cell lines described herein [ 9], which supports the use of gamma-secretase inhibitors in clinical trials [ 47].
This approach was used as published data documenting the doses associated with 20% cortisol suppression for each inhaled corticosteroid formulation, using the same methodology, were not available.
Gene expression profiling of ES cells after suppression of Sox2 was carried out using the same protocol (Tet-inducible transgene cell line 2TS22C; the same array platform) as the experiment on Pou5f1 [ 16].
However, there was a suppression of self-right neurons in position A1 and an absence of this suppression in position A2, even though M2 was still using the same tool.
This level of suppression is superior to that previously reported for anastrozole using the same methods: 89.0, 83.4, and 72.9%, respectively (Geisler et al, 2001).
Using the same methods, we investigated whether excess or insufficient CycD/Cdk4 activity is causing cell death by suppression of autophagy (Wang et al., 2009).
"Using the same model.
Using the same process, continue.
Find the carbohydrate using the same method.
DW-MRN uses the same principles as diffusion-weighted whole-body imaging with background body signal suppression [ 8].
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