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Electrophoresis was performed using the same solution at 18 V and 300 mA for 18 min.
On the other hand, reproducible cyclic voltammograms were obtained for 1 mM Ru3 CO 12 solutions, even when using the same solution and platinum electrode surface for a week.
When multiple recordings were made for a sensillum using the same solution, the rate of spiking was averaged over all the recordings made for each GRN class.
Slides were then washed in phosphate-buffered saline (PBS) for 5 min and immersed in a freshly prepared alkaline buffer (pH 1 mM EDTA and 300 mM NaOH, pH > 13) for 20 min and the electrophoresis was carried out using the same solution conducted for 20 min at 25 V and 300 mA.
Proteins were first eluted with 0.1 M glycine, pH 2.8 and neutralized with 100 mM TRIS-HCl, pH 8.0, followed by a second elution using the same solution, a third elution using 10%1,4-dioxane1,4-dioxaneisher Scientific, D111-500), and a fourth elution with elution buffer (50 mM TRIS-HCl, pH 6.8, 100 mM DTT, 2% SDS) for 40 min at 65 °C.
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"We also need to take a new approach to things that are not working, like using the same solutions for all school problems," Mr. Harkin said.
At the same time, with a language development, it seems that we are stuck in the past, for years using the same solutions.
Mann-Whitney U test was used for statistical analysis Care was taken to stain control and β2−/−/γ3−/− cells in parallel using the same solutions as well as to acquire the confocal images with exactly the same settings of the microscope.
Müller cells from wild-type and β2−/−/γ3−/− mice were stained in parallel, using the same solutions, and images were acquired using identical settings of the confocal laser scanning microscope.
Each μDCell was tested using the same solutions of fluorescein within the range 0.01 500 μmol L−1.
Controls and mutants were stained simultaneously using the same solutions, in the same Eppendorf tubes or glass vials, and imaged on the same days using identical microscope settings.
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