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All isolates displayed a highly similar antigenic pattern when they were assessed using the same serum.
Each testing round was done blind using the same serum samples at IAH.
Individual clones identified in membranes were plated for secondary and tertiary screenings using the same serum to obtain monoclone and retest for serum reactivity.
Overall, the Wantai assay found a much higher seroprevalence of anti-HEV antibodies compared with the WRAIR assay, using the same serum.
In contrast, in our previous report using the same serum samples [ 14] only 56% of the patients expressed one or more autoantibodies.
The phage plaques on the agar plates which corresponded to positive plaque images on the X-ray film were cored out and subjected to secondary and tertiary screenings using the same serum.
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In the previous GBNP study that used the same serum samples as the present study, antibodies to Leptospira ssp.
We then used the same serum samples to evaluate the diagnostic sensitivity and specificity of different combinations of the individual autoantigens in this differentiating panel of six biomarkers.
The Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) recently published an equation for estimated glomerular filtration rate (eGFR) using the same variables (serum creatinine level, age, sex, and race) as the Modification of Diet in Renal Disease (MDRD) Study equation.
Using the same methodology, serum-starved HFF cells were exposed to 1000 particles per cell of virions lacking either gD, gB or gH and RNA was extracted for cDNA synthesis immediately (0 h) or after 6 h.
Values for the testing and symptomatic sets were normalized using the same HK serum-miRNAs.
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