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Immunostaining using the same sera in tissue culture cells showed an enrichment at centromeres (Earnshaw and Rothfield 1985; Earnshaw et al. 1986; Valdivia and Brinkley 1985).
To further support our proteomics findings, we measured by ELISA a subset of deregulated proteins using the same sera in our training set and in an additional validation set of 50 women.
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Furthermore, Western blot analyses of P. falciparum-schizont lysates using the same hyperimmune sera showed strong reactivity with protein bands of 63, 54, 51 and 48 kDa, which are close to the molecular weight of MSP-2 (∼69kDandand its cleavage fragments.
Moreover, no staining was observed using the same anti-sera to blot cell lysates from the parental cell lines not transfected with ST6GalNAc I and therefore not expressing STn (data not shown).
Seventeen weeks after the 2nd booster the mice were re-immunized using the same protein concentration and sera were collected 10 days later.
To clarify further the specificity and sensitivity of serum sialic acid monitoring, analyses of sialic acid by the thiobarbituric acid method and acute-phase reactants by radial immunodiffusion were made using the same malignant melanoma patients' sera.
Polyclonal antibodies were purified from sera using the same GST fusion proteins.
Additionally, these 10 biomarkers could distinguish AD from breast cancer and Parkinson's disease sera using the same microarray and algorithm approach.
The IA-2A index for each sample was calculated using the same JDF standard serum and control sera that were used in the GADA assay.
Using the same methodology, 11 out of 21 (52.4%) sera from women with precancerous lesions were able to induce apoptosis in more than 30% of Jurkat cells (Fig. 1b).
Stratification means that sensitivity values have to be calculated at a predefined specificity (mostly 98% or more) using the same cohort of RA patients and disease control sera.
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