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Previous studies using the same sequencing technology also report statistics consistent with a low level of error once the sequences have been appropriately screened for quality [7].
In addition, having fewer amplicons also helps in obtaining deeper coverage using the same sequencing throughput.
An uninoculated whole root single replicate control was also sequenced using the same sequencing protocol.
In addition to this, an empirical prior can be obtained form previous sequencing experiment using the same sequencing technology.
Our mean assembled transcript lengths are greater than or equal to those reported in similar studies using the same sequencing technology [ 80, 81].
Nevertheless, our de novo assembly of the T. absoluta transcriptome was comparable in size with previously published assemblies sequenced using the same sequencing platform.
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Functional imaging data for the single-item-level event-related Experiment 2 were comprised of eight functional runs, each containing 209 continuous whole-brain functional volumes using the same sequence parameters as the block-design scans.
These blocks are then scrambled chaotically by using the same sequence RS through key SK.
Using the same sequence, alignments were obtained by the Clustal X program (1.83) [32].
For that, we performed "stability simulations" using the same sequences (see materials and methods).
Sequences that are incorrectly classified by all considered methods were identified and localized in sequence space using the same sequence distance, clustering and phylogenetic analysis.
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