Intracellular antigens were stained in suspension using the same primary and secondary antibodies as used for immunostaining.
No specific staining was observed with the rabbit and goat non-immune isotype IgG controls using the same conditions and secondary antibodies (negative control; Fig. 4E).
After treating 30 min to 1 hr in Odyssey blocking buffer, the membranes were then incubated for 1 hr at RT with 1 10,000 of anti-PGK using the same IgG-HRP-secondary antibody as described.
Despite the absence of detailed evaluation it is clear that these investigations should be accessible to primary care, using the same referral guidelines as secondary care, although in the short term this may be dependent on availability and the allocation of resources.
When serial tumor sections were stained for CD31 and CD105 and visualized using the same fluorescent dye-conjugated secondary antibody, no detectable difference in the localization pattern was found between CD31 and CD105 (Supplemental Fig. 3).
We also re-run our primary analysis using the same data as for the secondary analysis.
Furthermore, it should be noted that total sum-rate capacity is the summation of primary sum-rate capacity and per beam secondary sum-rate and we apply the IA technique to get better spectral efficiency using the same frequency resource by primary and secondary systems.
If Tsarnaev is found guilty on any of the capital charges, a secondary phase using the same jury will be triggered, in which the jury – not the judge – will decide whether to sentence Tsarnaev to death.
This set of parameters should also be valid for similar scenarios (secondary scenarios) using the same frequency.
