The third step was carried out during a replicate chromatographic run using the same RP-HPLC conditions.
All reactions were monitored by analytical RP-HPLC (0 100 % CH3CN/0.05 %TFA in H2O/0.05 % TFA over 40 min), the compounds were purified by semipreparative RP-HPLC (using the same gradient) and characterised by mass spectrometry.
After the reaction reached completion by LC-MS, the compound (Folate-Lys-NHCO-Dylight680B-Cysteine-S,S-promofluor750) was isolated by preparative RP-HPLC using the same condition, and pure fractions were lyophilized to give the NIR folate-DA chemical.
However, for sample collection, Chromolith SemiPrep RP-18 endcapped 100 10 mm was used to collect eluted sample using the same developed method.
(b) Project the use of wood in the CP by using the same % of HWP commodities (sawnwood, wood-based panels, paper and paperboard) as documented for the RP. .
Computed (DNS/EEM) and measured (EXP) equivalent radii RP and RS, mean stretch k and consumption velocity SC, as well as sample front shapes, are compared using the same post-processing procedure.
Using the same process, continue.
Thus, to address this question, we used conventional ChIP to measure the levels of H3K36me3, H3K4me3, and H3K79me3 at the intron and 3' exon of the same RP genes used to analyze H2BK123ub1.
In many cases, different RP paralogs from the same RP families displayed differential expression.
The 80 RPs are encoded by a total of 249 genes; thus, several paralogous genes encode the same RP [ 1, 4].
For the recognition of RP-sites we used the same computational algorithms as published by Burnette et al. 2005 with the same 80% scoring threshold for counting the number of RP-sites.
