Sentence examples for using the same restriction from inspiring English sources

These overhangs are very useful in cloning because the unpaired nucleotides will pair with other overhangs made using the same restriction enzyme.

In addition, the design of restriction enzyme cutting sites using the same restriction enzyme (Nt.BbvCI) in hairpin probe and MB respectively, improved reaction efficiency cost-efficiently.

The β-galactosidase encoding gene (lacLM) of pTM51R was excised with Bgl II and Spe I, and replaced by the multiple cloning site (mcs) from pNZ8150 ( Mierau and Kleerebezem 2005 ) using the same restriction sites; the resulting plasmid was designated pVK51.

The PCR-fragments were cut using restriction enzymes XhoI and BamHI and ligated in pHA-vA by using the same restriction sites.

The PCR product was digested with XhoI and BamHI and inserted into the pEGFP-N1 Vector (BD Biosciences Clontech) that was digested using the same restriction enzymes.

The amplified genes were inserted into pET22b vector using the same restriction sites to generate a C-terminal his-tag fusion protein.

Even if an appeal is successful, the home secretary can immediately issue another control order, on the same grounds and using the same restrictions.

pMU3KO is a substitution of the In711 anti-sense RNA with a random sequence of RNA created by overlapping PCR and cloned using the same restrictions sites above.

The amplified DNA fragment was then cloned in frame with a myc tag into the pcDNA3.1-Myc expression vector using the same restrictions sites as above.

Using the same restrictions we also fitted a log-binomial regression model with elevated TFS as the dependent variable and abdominal pain, fatigue, myalgia and arthralgia (possible symptoms of haemochromatosis) as independent variables.

For mapping significantly associated loci to linkage groups (LGs), we used RAD sequences generated from Miller et al. (2012) to match identical loci in our data that used the same restriction enzyme (Sbf1).