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Using the same reads simulation procedure and the same error measurement to the former simulation, we assess the performance of PoolHap on all genes of A. thaliana with more than one isoform.
Motivated by the significant difference of the detected taxonomic abundance between the results generated by MetaphlAn and CensuScope using the same reads set, we performed an additional analysis on a relatively small but complete real metagenome dataset (SRS011236).
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Of the highly expressed miRNAs common to both parasites, five (lin-4, mir-45, mir-50, mir-71, mir-228) are shared with C. elegans, where all except mir-50 are also abundant (using the same read number criteria) [ 42].
In essence, the long march technique combines the desirable aspects of both shotgun sequencing and directed primer walking to produce substantially greater coverage within the same number of reads and using the same read length.
Using the same read-mapping approach as described above we defined a 5,190,533 bp core genome and phylogeny for the subset of 13 isolates from Benin and Ghana.
In fact in a nationwide sample, 64% of ELL teachers used the same reading materials for their English proficient students and their ELLs, despite the great disparities between these two groups (Moss & Puma, 1995).
We used the same read mapping approach to detect variants of the rps2 – rpoC2 intergenic spacer across Asclepiadoideae that could correspond to the homologous region of the mitochondrial genome if present in species other than A. syriaca.
Below we provide a demonstration of how users can apply CensuScope to speed up their metagenome analysis with other pipelines using the same synthetic reads that were used for CensuScope testing as shown in Table 2.
The DNA fragments missing from the reference assembled molecules but present in the genome map were readily identified among the de novo assembled contigs developed using the same raw reads.
The genomic reads were aligned to the KEGG genes database using the same parameters, read counts for KEGG orthologues summed and normalised to the total number of hits.
To increase the completeness of the proteomic analysis and to account for deficiencies in the Oasis assembly, we conducted X! Tandem searches against three other protein databases; (1) ESTScan predictions from a transcriptome assembled from the same reads using Trinity [ 25]; (2) all Cnidaria proteins from the GenBank non-redundant protein database; and (3) the complete SwissProt database.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com