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Using the same quantification and calculation methods as described in the legend of Figure 2, the copy numbers of two OATP1B3 mRNA isoforms in each sample were separately determined.
Remark that using the intensity values amounts to using the same quantification method as in ArrayGauge.
Results for these experiments were obtained using the same quantification method previously described using the other cell lines.
The protein expression level was estimated using the same quantification method as for mRNA expression level, as compared to MCF7 cells.
Importantly, we confirmed our observations using an independent RNA polymerase III subunit, Rpo31 GFP, in gpn2 -2 and gpn3 -1 mutants using the same quantification scheme.
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Analysis of PCBs in the SRM using the same extraction and quantification as the samples (SI Figure S1) resulted in a mean difference of 6 ± 17% between the NIST certified or reference values and our measured values for 22 congeners.
To assess the biological significance of the residual 10% we used precisely the same quantification method to analyse co-localisation between transferrin-alexa-647 and transferrin-alexa-546, after mixing and adding to cells for simultaneous internalisation.
Paired measurements, which are two consecutive measurements using the same method, permit the quantification of the change within the measured parameter, for example, ΔCO.
A second set of analyses measured the reliability of the registration and quantification using the same approach on longitudinal data acquired in 69 healthy adults at a single field strength, 1.5 T, at an interval < 2 years.
Neurofibrillary tangles in brain tissues from young and old fishes were quantified by analysis of confocal images acquired using the same procedure as for lipofuscin quantification.
The software calculates the interocular distance based on the head model, the ocelli, and the eye edges, thus using the same landmarks as in manual quantification.
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