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(Pcombined = 0.00012) While we have concentrated on multiple crosses using the same progenitor strains, it would be simple to extend this method to include crosses in other strains or, for that matter, other phenotypes measured in independent crosses.
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We use the same progenitor term used in Lapan and Reddien (Plos Genetics 2011) and Lapan and Reddien (Cell Reports 2012) describing the sp6-9 + and ovo + cells as eye progenitors, respectively.
To further study this behavior, we systematically explore the system by varying γ w and γ B, which are reciprocal of the EC50s, a measurement of strength of feedback for Wnt and BMP, using the same localized initial distributions of progenitor cells.
The researchers then differentiated the cells into neural progenitor cells using the same techniques that guide the differentiation of embryonic stem cells.
In this report we show that iPS cells, generated with the combination of OCT4, SOX2, NANOG and LIN28, can be directed to a retinal progenitor fate using the same protocol as we developed for hES cells.
Sub-genome specific amplification was not detected from diploid progenitor species using the same set of primers.
We have previously examined the expression of the myogenic regulatory factors (MRFs) myoD1a, 1b, 1c, myog, Myf5 and MRF4, MEF2A and a marker of myogenic progenitor cells (Pax7) using the same cultures [48].
Using the same method, we estimated that O. sativa and O. glaberrima progenitors diverged between 1.2 and 0.8 Ma (fig. 2).
In this study, we used PDGFR α, which is conserved in humans, and demonstrated that cells equivalent to mouse PDGFR α+ mesenchymal progenitors can be identified in human muscle using the same marker.
We now report the first description of bovine mammary progenitors and stem cells defined by functional growth endpoints using the same in vitro and in vivo xenotransplant systems previously developed for their human counterparts.
Together, these results indicated that we produced neural progenitor cells similar to those we created in a previous study using the same protocol (Rasmussen et al., 2011).
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