qRT-PCR curves were drawn using the GraphPad Prism software (GraphPad Prism, Inc., US). p values were calculated using the same GraphPad Prism software.
By recombining the two phase-encoded beams using the same Nomarski prism, the differential phase information is converted into an intensity modulation.
After recombining the phase-encoded beams using the same Nomarski prism, the spectrally-encoded beam is directed to a spool of optically-pumped dispersive fiber (with dispersion value of −1373 ps/nm) via an optical circulator to perform amplified dispersive Fourier transformation (gain of ~20dB).
All statistical analysis were performed using the same software (GraphPad Prism, La Jolla, California, USA).
The PCR amplified fragments for each of the strains were then subjected to electrophoretic separation on a 5% acrylamide gel on an ABI Prism automated DNA sequencer and scoring of the fluorescent markers was done using the same DNA analysis workstation (ABI Prism 3100 DNA sequencer).
All samples, in which variant bands were detected, as well as two normal bands per exon, were sequenced using the same PCR primers and ABI Prism 310 Genetic Analyzer (PE Biosystems, Foster City, CA, USA).
Cylinders were also cured using the same condition as used for the prisms.
Another outstanding demonstration was reported by Murray and co-workers who prepared highly uniform hexagonal NaYF4 Yb3+/Er3+ nanoparticles with controlled sizes and morphologies (spheres, nanorods, and hexagonal prisms) by using the same synthetic strategy.
Sequencing was performed with BigDye Terminator 3.1 (Applied Biosystems) on an ABI PRISM 3130×l sequencer using the same primers as used for PCR amplifications.
PCR steps included an initial denaturation step at 95°C for 3 min, followed by 45 cycles at 95°C for 30 s, 48°C for 30 s, and 72°C for 45 s, and a final extension step at 72°C for six min. PCR products were gel-purified with the QIAquick Gel Extraction Kit (Qiagen) before sequencing on an ABI Prism 3130 XL Genetic Analyser Applied Biosystemss) using the same primers.
