Sentence examples for using the same polymerase from inspiring English sources

These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) programme.

PCR primer walking used to confirm the results for the second agr locus were performed using the same polymerase as above, with annealing temperatures of 55°C.

This mutation rate is higher than previously reported in hORFeome v1.1 (one mutation every ∼ 35,000 bp) using the same polymerase, but that analysis was limited to only 70,000 nt [23].

Genomic sequences containing the full introns and exons of the aqp0a1, -0a2, -0b1, and -0b2 genes were amplified from genomic DNA by PCR using the same primers and polymerase used for full-length cDNA cloning.

cDNA was synthesised using 0.5 µg total RNA, primer and Superscript II reverse transcriptase (Invitrogen) and amplified by PCR using the same primer, Taq DNA polymerase (Promega) and 2.5 mM MgCl2.

The first strand cDNA was then synthesized to double-stranded DNA using the same primer and Klenow DNA polymerase (Takara, Dalian, China).

Short DNA fragments were also generated from a range of templates with a whole genome amplification kit based on activity of the Φ29 DNA polymerase, using the same methodology.

To establish that the compounds did not inhibit the 3Dpol activity by merely chelating with the nucleic acid, we monitored the effect of 20 µM of the inhibitors on the DNA synthesis by two unrelated nucleic acid polymerases, using the same template-primer system (poly-rA/dT18).

HD-PTP deletion variants were PCR amplified with Pfu DNA polymerase (Promega) using the same template pMObsr-Flag-HD-PTP.

Eight differentially expressed genes were chosen for quantitative polymerase chain (qPCR) using the same RNA samples that were used for the microarray.

This possibility was eliminated by demonstrating that the inhibitors did not affect the polymerase activity of two unrelated polymerases, HIV RT and KF, using the same nucleic acid as substrate (poly-rA/dT18).