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To further optimize this animal model, we tested other conditions for SS disease induction using the same peptide, and switched to SJL/J mice, a well established autoimmune prone animal model [9], [10].
This previous polyclonal anti-progerin did not detect progerin in normal tissue, and to rule out the possibility of the antibody being of low affinity or that the recognized epitope might be masked, we generated a rabbit monoclonal antibody using the same peptide as antigen as described in Materials and Methods [30].
The anti-SGTA antibody was raised against the following peptide (CRSRTPSASNDDQQE-COOH) and affinity-purified using the same peptide conjugated to Sulfolink coupling resin (Thermo Scientific).
Polyclonal antibodies were purified using the same peptide bound to Sulfolink Coupling Gel (Pierce) according to manufacturer's instructions and dialyzed against PBS before storage.
and sodium azide (3.0 equiv .. 26 Ahx and PEG-based spacer systems were synthesized using the same peptide coupling methodology as described for the synthesis of the linear RGD peptide (see above).
Moreover, in a separate phase I clinical trial using the same peptide vaccine, the production of anti-MUC-1 circulating antibodies was detected in patients with inoperable PC, although no significant impact on survival was discovered [ 103].
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Immunization of pGL261-glioma-bearing mice using the same peptides was ineffective, demonstrating that the immune response induced by peptide vaccines specifically targets mIDH1-expressing cells.
An even more important comparison of our findings with the Kd values observed for F1 TnC using the same peptides (26) should be conducted.
Thus, it is reasonable to speculate that the FREX mechanism can be transferred to other FN3 monobodies by using the same peptides and packing mutations employed in this study, with minimal additional optimization.
The first comparison was among studies using the same individual peptide (B type natriuretic peptide, NTpro-BNP, MRpro ANP) according to the three threshold categories.
The currently resolved substrate-bound 3D structures have typically been obtained using the same inhibitor peptide (PKI).
More suggestions(15)
using the same neo-realist
using the same methodology
using the same building
using the same number
using the same language
using the same tool
using the same type
using the same explanation
using the cyclic peptide
using the synthetic peptide
using the same playbook
using the corresponding peptide
using the same formula
using the same air
using the same hormone
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