The intron amplified using the same PCR recipe as above and PCR program- ii.
RT reactions that were conducted without primers (-p) also generated measurable amounts of PCR products using the same PCR primers used to detect sense and antisense MYH7 RNA (Fig 1).
mtDNA with TTGE banding pattern abnormalities then underwent direct DNA sequencing using the same PCR fragment; detected mutations underwent repeat PCR and sequencing for confirmation.
The amplification reaction was performed using the same PCR profile as mentioned above and the PCR amplicon was cleaned as mentioned above.
For RNA normalization, an 18s rRNA PCR was performed for each cDNA using the same PCR reagents except for the primers and probes which were the Eukaryotic 18srRNA Endogenous Control (Applied Biosystems).
Each DNA fragment band originated from the PCR-SSCP technique was scrapped and individually reamplified using the same PCR reaction condition.
After the extraction of organelle DNA, PCR products were re-amplified with the conservative primer pairs by using the same PCR programs as mentioned above.
The PCR products were purified using the Wizard SV gel and PCR cleanup system (Promega) and then sequenced using the same PCR primers.
Furthermore, blood DNA samples collected from 767 cattle, grazing on the same pastures where the ticks had been collected, were negative for Theileria sp. (sika 1), using the same PCR assay.
All hANT knock-in DNA fragments were constructed using the same PCR strategy described above.
SNP analysis of these 6 extractions were compared to previously analysed European SNP data using the same PCR assays and were found to be the same.
