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Fluorescence was collected using the same objective and guided to a confocal pinhole to reject out-of-focus light.
The laser beam was focused onto the sample through an apochromatic 60×, 1.2 NA water immersion objective, and the emitted fluorescence was acquired using the same objective.
Various combinations of the non-lethal gene deletions leading to about 111,628 different double knock-outs were generated and tested with FBA using the same objective function.
Finally, the backward-directed SHG was collected in an epi-fluorescence configuration, using the same objective for focusing and for collection.
To distinguish eGFP-centrin1 intensities in both experiments, reference images were taken at the end of the experiment, as three-dimensional stacks of 40 × 0.3-μm steps and a high-exposure times using the same objective and camera.
Note that ℓ1-regularization logistic regression can be obtained from KELLER: we only need to apply a uniform weight w(t i )=1/ n to all observations and estimate a single network using the same objective as Equation (5).
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Moreover, it uses the same objective function as defined in [11].
Meanwhile, [11] directly integrates the coordinates into the transmission likelihood of the NetRate and thus uses the same objective function as the optimization target.
Since they both use the same objective function to choose labels, ICM and modified MASP should have similar labeling result.
Recent UK studies in 3 5-year-olds 14 and 11–12-year-olds 25 which have used the same objective methods of PA measurement have found similarly low levels of PA with adherence to the 60 min/day recommendation of MVPA being achieved in <5% of their samples.
We used the same objective criterion to identify light-responsive units in both in vitro and in vivo recordings that firing rate within 4 s of the start of a 2-s pulse fell >2 SDs outside mean of baseline firing prior to light exposure.
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CEO of Professional Science Editing for Scientists @ prosciediting.com