However, these promising data could not be reproduced in another study using the same mutant mouse strain [ 31].
Compared to the previously reported single-chain ZFN targeting m.8993T>G, our current design is superior in shifting heteroplasmy while using the same, mutant-specific DNA-binding zinc finger peptide (NARPd).
In essence, we use the same mutants to identify and confirm the primary "hot spot" of interaction, as well as proximal sites that may be not indispensable for binding yet allow the formation of a stable covalent adduct.
Interestingly, a previous report that used the same mutant mice showed a deficit in late LTP and long-term memory and established that Egr1 is required for these functions [32].
Though not essential we suggest the following – either for this paper or for a discussion of future directions: reconstitute pol V Mut with a RecA mutant that can no longer bind ATP (e.g., less conservative mutant) and determine if it still binds etheno-ATP, and to use the same mutant in ATPase assays.
The second method detects purR mutants selectively by using the same mDEL1 mutant (TT26174) grown to saturation at 30° in 2.0 ml NCE with 0.2% glycerol and the appropriate supplements.
However, most beta-lactam hypersensitive mutants such as rpmF, rimK, rplA, pgmB, dksA, phoP, surA, emtA, identified in a previous study using the same Keio mutant library [28] were able to form L-form colonies in this study.
This is in contrast to studies using the same P101L mutant expressed in Drosophila, in which aged flies showed characteristics of protein misfolding and clear phenotypes of neurodegeneration reminiscent of the GSS syndrome.
Different labs reporting different results using the same eat-2 mutants strain [18], [46] highlights the difficulty of performing epistasis analysis on a non-optimizable DR method such as eat-2 mutation.
The null model was defined as using the same input data (mutant, ChIP-chip, TFBS, and gene expression data) but only the overlap was calculated instead of going through the iterative procedure described on Figure 1.
