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Tissues from each stage were collected in two replicates pooled from 15 plants grown under the same conditions and using the same morphological markers as previously described.
Almost all Heterococcus species have been described using the same morphological approach, we have examined all existing authentic culture strains, and we find that morphological species descriptions are inadequate for this asexual genus.
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Morphological images using the same geometry as diffusion-weighted images have to be performed in order to compare morphologic and functional images, allowing exact localization of the lesion and avoiding necrotic areas for region of interest delineation.
If the method of analysis was the main methodological driver of phylogenetic incongruence, then: (i) morphology-based Bayesian phylogenies should reduce conflict with molecular phylogenies inferred using the same methods; and (ii) the MP morphological phylogeny should share more nodes with the MP molecular phylogeny than with the ML or Bayesian molecular phylogenies.
In contrast, more recently, a phylogenetic analysis combining both morphological, and molecular data (using the same genes as in [ 21]) recovered Neogastropoda as monophyletic [ 5] stimulating the debate.
To confirm that cortactin was indeed knocked-down in experimental testes, Western blots were run on whole testis lysates from animals using the same reagents as those used for the dye morphological studies.
Although no morphological alterations were detected, this procedure was important to understand and confirm the inhibition of the hyphal growth and conidial germination, using the same incubation conditions.
Additional morphological information can be provided by second-harmonic generation (SHG) microscopy [ 19– 29], which can be combined with TPF microscopy using the same laser source.
The fact that a relatively high number of morphological features were used for each pairwise classification (i.e., 53, 80, and 99) compared to previous studies using the same method but achieving better between-group discrimination reinforces this notion.
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