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Images of the whole mounts were acquired with an Olympus digital camera using the same magnification and lighting conditions.
For each treatment and each time point, TUNEL-labelled nuclei were counted in coral sections obtained from 6 coral branches (6 sections were analysed and averaged per coral branch), using the same magnification (x40) and compared to the total number of host cells in the same image field to obtain a percentage of TUNEL-positive host cells.
All tissue images have been captured using the same magnification (4x objective; 10x on ocular).
Whole mounts of IKMV and control transplanted glands, treated with dox for 3 weeks, were imaged at the same session and using the same magnification.
The number of GFP-LC3 dots was determined by manual counting in 5 fields, and nuclear number was evaluated by counting DAPI-stained nuclei in the same fields using the same magnification.
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All digital images of tissue sections were captured using the same microscope magnification.
The organisms were investigated using exactly the same magnification (× 40) in order to allow data comparison.
The a-MVD is determined using the same grid and magnification (200×) as for h-MVD and calculating the mean of the vascular counts obtained in at least 10 15 random fields for each tissue section.
All images were exposed using the same exposure time under the same magnification.
Fluorescence was recorded using a confocal Zeiss LSM 710 microscope (×63 magnification), using the same laser intensities and detector gain for all slides in the experiment.
Confocal images were captured using the same parameters setting: equal optical magnification (60×) and electronic zoom (2×), identical laser potency (5%), identical photodetector gain (HV 480 V), identical scanning speed (12 µs/pixel).
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