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The ex vivo images were taken by using the same Maestro system as used for in vivo studies.
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Tumors on the peritoneum were analyzed for Cherry-Red flusingcence using the Maestro imaging apparatus (Maestro 2, Cri; Perkin Elmer) with an exposure of 150 nm.
Readouts were taken on the same days as the treatment, using the Maestro CRi optical imaging system (Maestro CRi Inc., Waltham, MA, USA).
Fluorescence images were obtained using the Maestro system and processed with the Maestro software 2.8.
Mice bearing xenografted tumor (3 weeks after the cell injection) were imaged using the CRi Maestro imaging device.
Fluorescence of different modified NPs in nude mice was obtained using the Maestro EX (CRI) in vivo optical imaging system.
Finally, the docked poses were visualized using the Maestro 10.1 graphical user interface.
Both ligands were built using the Maestro (version 9.3.35) Edit/Build panel.
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