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Aside from comparing the basic specs, we shot two scenes with each camera, using the same lighting and "Auto" mode without flash.
The images, acquired using the same lighting and imaging parameters, were adjusted to the same threshold and a histogram was produced for each picture.
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All images were segmented using the same light threshold, mask smoothing and object size filters.
Here, using the same light source as that used for behavioural testing, we found a clear, melanopsin-driven c-Fos induction in medial visual/retrosplenial cortex (Figure S1).
Briefly, all images from a given experiment were acquired in a single session using the same light intensity and filter settings with the white balance of all images standardised.
High light treatments (500 and 1000 μmol photons m-2 s-1) were given using the same light source and were started immediately after the diurnal dark period.
In other ongoing studies using Advillin-cre/ChR2 and trkB-CreER-ChR2 mice we can evoke action potentials in LTMRs directly using the same light stimulus.
The rinsed samples were then postcured for 10 min using the same light source and intensity without the photomask and under ambient conditions to maximize the monomer conversion.
Plants on plates and trays were grown in growth chambers using the same light and temperature conditions as described earlier by [ 30].
However, in ongoing studies using Advillin-cre- and trkB-CreER-ChR2 mice, action potentials can be evoked in Aβ and Aδ LTMRs using the same light stimulus (not shown).
X-ray film brain images were digitally scanned from a dissecting microscope connected to a SPOT-III CCD camera using SPOT imaging software (Diagnostic Instruments, Inc .. Care was taken to use the same lighting settings across all images used for quantifications.
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