Similar results were found when F2 hybrid mice of susceptible BALB/c and resistant strains were immunized and tested for arthritis- or spondyloarthropathy-associated QTLs using the same antigen, immunization protocol, and scoring system (visual and histology) and when MHC- and age-matched animals were housed in the same room, occasionally for more than half a year [ 9, 11, 23, 24, 29, 66].
The Ty21a oral typhoid vaccine capsules are licensed for adults and children above 6 years using the same dose and immunization schedule and large Ty21a field trials in children used the same dose and regimen as in adults [13].
The method also removes the bias of using the same material for both immunization and immunological assay which occurs when ELISA is used.
Comparable responses were observed with IM immunization using the same virus dose (Figure 2A).
To evaluate long-term protection, the challenge of mice was done 5 months after the last immunization, using the same dose of R. equi.
The specificity of the generated anti-hRasGRP4 antibodies was confirmed by absorption assay using the same peptide as used for immunization both in immunoblot and in immunohistochemistry using lysates of epithelial cell line HEK-293 (line CRL-1573; American Type Culture Collection) transfected with expression constructs encoding hRasGRP4 with the C-terminal V5 epitope tag (data not shown).
Two booster immunizations using the same antigen emulsified in incomplete Freund's adjuvant were carried out on days 14 and 28.
Some Canadian health regions chose to collect pandemic (H1N1) 2009 influenza immunization data using the same system implemented during the annual seasonal influenza vaccination campaign while in other areas, new methods were adopted.
Immunization of pGL261-glioma-bearing mice using the same peptides was ineffective, demonstrating that the immune response induced by peptide vaccines specifically targets mIDH1-expressing cells.
Using the same specific primers set as before the final immunization, the amplification of the PCR products increased and reached optimum 10 days after the last immunization, where all primer pairs were strongly amplified compared to the other days (see Additional file 1).
More importantly, using the same baseline comparator of "no vaccination" with which other immunization programs are commonly evaluated allows for comparison not only of current and prospective PCV programs, but also of PCVs against other immunization programs that will ultimately compete for limited financial resources.
