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All slides were imaged for the same duration of time, using the same gain and other imaging parameters.
and Metamorph software (Molecular Devices) using the same gain and intensity.
Background counts were collected with the laser off and the detectors on using the same gain and offset settings as used for collecting the time series.
All images were captured using the same gain and exposure settings, and upper and lower thresholds were set using the range indicator function.
For quantification of BRP and CSP aggregates, images were taken from axons that emerge from the ventral ganglion at segment A3, using the same gain for all genotypes.
Use of a specific binding peptide allows for the transition from normal to pre-malignant mucosa to be visualized with high contrast using the same gain on the PMT.
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In this proposed scheme, we force all the relays in one selected path P to use the same gain factor.
In this way, for the speech presence or speech absence in the frequency bins, they only use the same gain function to estimate clean speech from noisy speech, which limits their enhancement performance.
For data acquired from a given cell in channel mode, we collected pre-bleach and post-bleach images using the same detector gain, but the same detector gain was not necessarily used for CFP and YFP excitation.
For data collected from a given cell in lambda mode, we collected the pre-bleach and post-bleach emission spectra using the same detector gain, and we also used the same detector gain for both CFP and YFP excitation.
All images presented were from a single optical section, taken under 63X objective with using the same PMT, gain and offset settings.
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