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Although both NADH and FAD can be in principle simultaneously excited by two-photon absorption using the same excitation wavelength and spectrally separated on the detection, this would result in a reduced excitation efficiency for both fluorophores as well as in a higher cross-talk between the two signals.
The photoluminescence spectra measurements of CdS nanoparticles synthesized at 36 and 42 h were carried using the same excitation wavelength of 345 nm at room temperature (see Fig. 8).
Using the same excitation conditions, the integrated intensity of the 1,120-cm-1 peak corresponding to the v C-H ip-bend mode of Cy5, calculated from the measurements performed on fiv C-H ip-bendts (with a scanned length of 2 mm) on lines prepared with the WDE ink (AgNO3 concentration of 2.5 10-2 M, six passes, and 1,000 dpi), shows fluctuation lower than 20%.
It is important to note that we used laser settings to optimize the SHG signals over the DHPR signals when using the same excitation wavelength (880 nm) that might somewhat skew the resolution of the DHPR signals due to suboptimal quantum efficiency for the fluorescent dye.
It was not possible to quantitatively compare the FPs by FACS using the same excitation wavelengths used in confocal microscopy.
After exposure to ∼3 ppm ozone for 10 min, no discernible fluorescence could be detected from the sample using the same excitation and emissions wavelengths.
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In a first set of experiments, we used the same excitation energy for the three excitation patterns which, depending on the number of target cells in each experiment (from 5 to 11 target neurons per slice), corresponded to excitation densities of 0.6 2.2 nJ/µm2, 2.8 12.1 nJ/µm2 and 5.4 33.3 nJ/µm2 for the elliptic, shaped and anti-shaped patterns, respectively.
The tests were conducted using the same input excitation conditions (10 m/s2), frequency range of (50 150 Hz).
FAF was acquired using the same device (excitation at λ=488 nm, emission 500 700 nm).
Deposition of autofluorescent material was observed with epi-fluorescent light using the same microscope (excitation filter 485 nm, dichroic mirror 510 nm, barrier filter 520 nm).
Moreover, Zoladek et al. [ 18] did not report photodamage issues in their time-course Raman imaging studies of apoptotic cells over 6 hours using approximately the same excitation conditions as in this study.
More suggestions(15)
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