Sentence examples for using the same enzymes from inspiring English sources

Then, Aoxyn11A G21I was inserted into pPIC9K vector digested using the same enzymes, followed by transforming it into E. coli DH5α.

For lily expression, the same construct was subcloned into pBS SKII+ under the control of the zmC13 promoter using the same enzymes [35].

The ∼900 bp osm-10 upstream promoter region was isolated from CR142 [72] using the same enzymes and was inserted into the SphI/BamHI sites upstream of the dop-3 genomic clone in the remaining fragment of pCL35.

A fragment containing the rpoB-promoter region, the rpoB-leader, and the Shine-Dalgarno and startcodon was PCR amplified using the primers Prpofwd and PrpoRev2 (5'-GCGGATCCTGCCAAACAGATTCAC-3'), digested with EcoRI and BamHI and ligated into pLUG520 where the PrpoB-spa-fragment had been removed using the same enzymes.

PCR products were digested with PstI and NotI and cloned in pBI880 digested using the same enzymes.

They proposed an alternative pathway, the Kandutsch Russell (K R) pathway, in which the Δ24 bond of lanosterol is reduced and the conversion of dihydrolanosterol to cholesterol proceeds via 7-dehydrocholesterol using the same enzymes as the Bloch pathway.

The vector YIplac211 was cleaved using the same enzyme.

To compare the hydrolytic performance between Acremonium cellulase (commercial origin) and cellulase produced from PS (PS origin), PS was hydrolysed using the same enzyme activity.

The DNA fragment containing the coding sequence of ALR2 (NM_001667), digested from ARL2-PET by NdeI and BamHI, were inserted into pPyCAGIP-Flag vector using the same enzyme sites.

In comparing the two miniantibodies with the commercial tTG-specific monoclonal antibody CUB7402, the results show that the percentage of enzyme activity inhibition is higher with the miniantibodies compared to CUB7402, using the same enzyme to antibody ratio.

In E. coli K-12, the O antigen that K-12 used to have is built on GlcNAc (Stevenson et al., 1994, J Bacteriol, 176: 4144) using the same enzyme that initiates ECA.