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The molecular weight cutoff probability distributions after fitting the solute exclusion data to a sigmoidal curve model showed that the composite PES membrane presented a sharper pore size distribution (based on the ratio of standard deviation to average MWCO) compared with two commercial membranes of MWCO ~ 400 500 Da: N30F53 and NDX (Fig. 5c), which were also tested using the same dyes.
Intracellular pH measurements were also performed using microscopic fluorescent ratiometry using the same dyes and wavelengths as in the cuvette experiments.
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This corresponds more closely to cellulose-based transparent sensor layers using the same dye chemistry (pK a of 7.71) (Mohr and Müller 2015) and indicates that for the strongly coloured washcloths, the high degree of colouration slightly decreases the observed pK a value (a phenomenon frequently observed for too large dye concentrations in optical sensors, see also Wolfbeis 1991).
DOI: http://dx.doi.org/10.7554/eLife.04630.007 Kinetic unwinding assays were also performed using the same dye-labeled dsRNA in the presence of an unlabeled duplex.
When serial tumor sections were stained for CD31 and CD105 and visualized using the same fluorescent dye-conjugated secondary antibody, no detectable difference in the localization pattern was found between CD31 and CD105 (Supplemental Fig. 3).
Consider using the same dolor dye for all of your hair.
Polyester can't be dyed using the same kinds of dyes that work well with natural fibers such as cotton; using these types of dyes will result in little or no change to your garment's color.
Make sure you are using the same brand of dyes, to avoid timing confusion when you color.
These results were corroborated using the same pH-sensitive dye in confocal experiments (Supplementary Figure S7).
DNA sequences were determined with an automatic sequence analyzer (Abi Prismtm 3130; Applied Biosystems), using the same primers and dye terminator cycle sequencing kit (Applied Biosystems).
A new silk sample weighing 2.45 g was iron mordanted, and dyed using the same procedure as above using individual dye solutions.
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