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We obtained similar results using the same drivers and the more potent of our two lam cDNA constructs (data not shown).
It is interesting to note, however, that human B-type lamins, when over-expressed by ubiquitous or muscle-specific Gal4 drivers, cause lethality, whereas over-expression of A-type lamins and emerin using the same drivers has no effect (Table S1).
Whereas expression of mutant forms of lamC have been shown to cause phenotypes in Drosophila [5], [6], overexpression of a wildtype lamC cDNA construct caused no noticeable phenotypes, and adults were fully fertile when using the same drivers and conditions that killed flies bearing lam constructs (data not shown, but see Table 2 for the GAL4 drivers).
Further confirmation of the role of neuronal parkin in locomotion was provided by using the same drivers to test for rescue in the park homozygote background.
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The Consumer Federation checked rates in most ZIP codes in America, it said, using the same driver profile in each area: a 30-year-old single female high school graduate who rents her home and has a clean driving record, a fair credit rating and a clerical job, and drives a 2000 Honda Civic 10,000 miles a year.
Using the same driver for both RNAi and the gene rescue construct is one possibility, but the cDNA may not function properly when expressed from an artificial promoter.
Expression of the pro-apoptotic gene rpr along with p35 using the same driver also produced extensive cell death in the anterior compartment (not shown).
We generated transgenic flies expressing the Slmb-3G86.32 fusion protein under a UAS promoter, and drove its expression using the engrailed (en -Gal4 driven -Gal4he same time we labelledrivere cells with mCherry using the sate driver.
In order to investigate the selectivity of the decline in visual function for G2019S, we expressed transgenes bearing seven further single amino-acid mutations in the dopaminergic neurons of the fly brain, all using the same driver (TH GAL4).
Western blot analysis (Fig. 1C) of hUCP3-high/da-GAL4 flies confirmed that the expression of hUCP3 was more than twice as high as in the highest-expressing single insertion transgenic line (line G) using the same driver.
It is also worthwhile to observe that, although participants did not always use the same drivers, they were very consistent in using their drivers of choice.
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