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The zebrafish map constructed de novo here and compared with a well-assembled sequenced genome demonstrates the rapid nature of this approach that took a few weeks of part-time effort, whereas a previous map using the same DNAs required several years to construct, cost 100 times as much, and had half the number of markers.
In order to validate the Methylation array Platform results, we used 3 additional techniques, EpiTYPER, Methylation Specific Multiple Ligation-Dependent Probe Amplification (MS-MLPA) and bisulfite sequencing, targeting a selected subset of 21 differentially methylated (DM) CpGs (DMCpGs) from 18 different regions and using the same DNAs studied by Methylation array Platform.
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Using the same DNA fingerprinting techniques used in paternity cases, scientists at the University of California at San Diego looked at the genetic differences between ants in Argentina and California.
Using the same DNA extraction protocol mentioned above, genomic DNA was extracted from 204 individuals (134 adults and 70 post-larvae).
Using the same DNA fragmentation in vitro assay described previously, we found that neither of these molecules could support Cas9 nuclease activity when complexed with any crXNA molecule (Supplementary Fig. 13b).
Using the same DNA-binding domains, we generated ZFNs that were screened in combinatorial pairs in cell-based extrachromosomal single-strand annealing (SSA) assays and in gene-targeting assays using stably integrated constructs.
PCR amplification using the same DNA primers as abovementioned was further performed for the confirmation of the JM83-AAT24, JM83-ATT24.
The reproducibility of the two DArT genotyping arrays was examined by independent assay using the same DNA.
When we mapped the 454 datasets for all of the isolates back to the finished sequence that was generated using the same DNA, we noted several putative SNPs that were common to all datasets (Table 4).
In order to confirm it we knocked down the expression of OD in BSF cells in a similar way as indicated for PCF trypanosomes and by using the same DNA construction.
The PCR amplified fragments for each of the strains were then subjected to electrophoretic separation on a 5% acrylamide gel on an ABI Prism automated DNA sequencer and scoring of the fluorescent markers was done using the same DNA analysis workstation (ABI Prism 3100 DNA sequencer).
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