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Individual colonies were picked up and cultured in liquid BG11 medium that contained glucose using the same culture conditions described above.
The pre-cultures were prepared using the same culture conditions as for shake flask cultivation.
These fermentations were done using the same culture media, carbon:nitrogen relation, operating condition and bioreactor.
When using the same culture medium, the higher growth temperature resulted in the shorter latency time.
Sub-culture was made using the same culture media to have pure culture colonies before identification was done by OmniLog.
In subsequent experiments we isolated O-acetylated and O-deacetylated diheteroglycan from E. faecalis type 2 using the same culture conditions.
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We have previously examined the expression of the myogenic regulatory factors (MRFs) myoD1a, 1b, 1c, myog, Myf5 and MRF4, MEF2A and a marker of myogenic progenitor cells (Pax7) using the same cultures [48].
I visited its northern factory with a friend who works for Icelandic Provisions, which uses the same cultures for its skyr — a type of yogurt culture which has been part of the Icelandic diet since the Viking days — from this very monopoly.
The selected cells were plated on coverslips placed in 24-well plates at 1 × 10 cells per well and cultured using the same cell culture conditions described previously.
Cells from positive wells were subsequently expanded in larger wells using the same stimulatory culture conditions but with more feeder cells.
Each panel of Figure 4 was generated using the same cell culture with sequential hypotonic challenges of different osmolarities.
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