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Typically assay controls are prepared at the same time as kodecytes using the same cells except they are incubated either with the FSL diluent (without FSLs) and ideally also with a benign FSL (of similar structure but with no activity).
Researchers need these for a number of reasons, such as having positive controls for assays, or to be sure they're using the same cells as another investigator whose work they want to replicate or expand upon.
As a compromise we compared the location of TbMyo1 and actin in separate localization experiments using the same cells and observed a broadly similar distribution of both proteins between the kinetoplast and nucleus consistent with their joint presence throughout the endocytic pathway (Fig. S6).
Genes without pseudogenes were classified for chromosomes, and the log ratio of the average of two experiments using the same cells was determined for each chromosome.
Interestingly, a clear increased of IL-6 and IL-8 by S. suis was reported in the literature using the same cells [ 13].
In this study, using the same cells, blocking these pathways with LY294002 and TAM67 enhanced cell proliferative arrest more than either agent alone, but neither agent alone nor their combination induced apoptosis.
Similar(52)
And as the case with Raptiva shows, the product can come out differently even using the same cell.
The injected pellet was incubated for seven days using the same cell culture medium, which was changed every two days.
In October, the authority began testing a smartphone payment reader using the same cell phone application available to riders on the Long Island Railroad and Metro North.
Using the same cell clones, we further performed Southern blot analysis to investigate whether off-target integrants were detected (Supplementary Fig. 8a).
The low cost imaging device compared favorably against the expensive commercial luminometer plate reader using the same cell based assay.
More suggestions(15)
using the same steps
using the same weapons
using the whole cells
using the hexagonal cells
using the viable cells
using the same strategies
using the washed cells
using the thyroid cells
using the same actors
using the algal cells
using the stable cells
using the standard cells
using the follicular cells
using the new cells
using the same plates
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com