Sentence examples for using the same cdna from inspiring English sources

We performed real-time qRT-PCR using the same cDNA set.

A significant difference (p<0.05) in confirmatory RT-PCR experiments using the same cDNA as for the microarray hybridization served as a stringent paramenter for microarray analysis evaluation.

Expression levels were then validated in a confirmatory experiment by real-time RT-PCR using the same cDNA hybridized on the array.

Real-time PCR results were expressed as mean values from 3 independent experiments using the same cDNA preparations and were normalized to GAPDH.

As a control, the expression of gene rp49 [32] was monitored by PCR using the same cDNA sample used for the analysis of yp2.

Using the same cDNA preparations to test for Anp32a and Anp32b mRNA expression, we found that these transcripts may be induced by deletion of Anp32e, particularly in thymocytes (Figure 1C).

In order to get a more precise comparison, we performed our study using the same cDNAs, to exclude all errors in the pre-analytic variables, such as sample isolation, sample volume, logistics and storage conditions, as well as important analytical variables, such as CTC isolation methodology, RNA isolation, and cDNA preparation steps [ 26].

Both E-cadherin and β- actin RT PCR reactions used the same cDNA synthesis.

To avoid discrepancies due to pre-analytical errors we used the same cDNAs throughout our study.

Bead-coupled cDNA was regenerated and two more rounds of hybridization were performed using the same subtractor cDNA.

cDNA samples were then quantitated with a NanoDrop spectrophotometer (Thermo-Scientific), using the same total cDNA quantities for each sample for Q-PCR assay on an ABI StepOne Plus instrument (ABI).