Elution was performed using the same buffer.
This step was repeated using the same buffer with 4% iodoacetamide in place of 1% DTT.
The effect of pH (from 3 to 9.5) on rPHY US417 activity was investigated at 65°C using the same buffer solutions reported in Farhat et al. (2008).
Active fractions were pooled, concentrated, and further purified with Sephadex G-25 column (1.5 × 28 cm) using the same buffer system.
Cells were incubated in the absence or presence of peptide venoms for 1 h, using the same buffer supplemented with 2.8 mM glucose.
Total protein extractions for all enzymatic analysis (activity and isozyme assays) were performed by using the same buffer except ascorbate peroxidase (APX) assays.
The pH stability using the same buffer solutions was also determined by pre-incubating enzyme samples at 35 °C for the period of 12 h considering PYT activity at zero time as 100%.
After that, the gel was washed twice (30 min each) using phosphate-citrate buffer (pH 6.0) containing 25% isopropanol, followed by twice washing (30 min each) using the same buffer but without isopropanol.
Lactobacillus panis PM1 cells grown to mid-exponential phase under microaerobic or aerobic conditions were harvested by centrifugation, washed with 100 mM phosphate buffer (pH 7.0), and the cells in pellets were then disrupted using sonication (three times for 1 min with a 3 min rest interval at output level 2, Sonifier 450; Branson, CT, USA) using the same buffer.
This process was repeated for each wire using the same buffer contained within the syringe.
