However, for results to be truly comparable to those reported by us, antibody titres among such patients would need to be assessed using the same assay, preferably performed at the same time and under the same laboratory conditions.
First, we performed dose-response analyses for these two compounds using the same assay described above (Fig. 2f).
Additionally the (S -3-HB negative control S -3-HBno reS -3-HBusinegativeame assay kit.
This agrees with previous data showing that Polβ overproduction increases (up to 4-fold) mutagenesis using the same assay [33].
Using the same assay, variable strengths of binding were observed for the SmTK3 constructs, depending on the extent of the N-terminal deletion (Fig. 7).
Previous studies support our findings, reporting variability between different assays as well as inter-laboratory differences using the same assay [16], [17], [18], [19], [21].
This result was similar to the enzyme activity measured in rXylanase expressed in E. coli (10.2 nmol/min/µg protein) and commercial Trichoderma viride xylanase (2.3 nmol/min/µg of protein) using the same assay conditions.
Using the same assay, BORIS transcript levels were undetectable in control BJ fibroblasts; but in BJ fibroblasts transduced with CMV-HA-BORIS adenovirus, were over 32,000 times greater than those in testis (Table 1).
Fasting serum samples were obtained during the research visit; they were measured in all participants using the same assay and under identical testing conditions at a UCSD clinical laboratory.
Using the same assay system, we also confirmed that EPRS, CtBP, eIF4E (Figure 1C), Squid (Figure S4B), and several other proteins found in our MS identification (proteins underlined in Table 2) are sumoylation substrates.
A further dilution series was then made from the undiluted RNA preparation (QIAamp method) to generate samples with Ct values of 15, 20, 25 and 30 and the expected level of FMDV RNA was confirmed using the same assay (data not shown).
