IHC results for HER2/neu vary with replicate testing using the same antibody clone and when alternate clones are utilized.
Each procedure was repeated a minimum of three times to validate the results and minimize any experimental errors using the same antibody stock.
A concentration of 0.1 ng l−1 gave a signal clearly distinguishable from the blank whereas the ELISA using the same antibody had a typical detection limit of about 1 μg l−1, which is four orders of magnitude higher compared to the presented biosensor system.
In line with others using the same antibody [13], we found that circulating CD4+ T cells were effectively deleted.
All combinations of Myc-, V5-, or un-tagged aSyn molecules were stained using the same antibody combination as described above.
Other studies performed in mice have also reported CSPG deposition post trauma using the same antibody [57].
However, immunodetection using the same antibody failed to localize syntrophin to the sarcolemma of the mdx muscle (Fig. 11 and Table 1).
As the control, no protein bands were observed using the same antibody pre-absorbed with an excess of recombinant VDAC2 protein (Fig. 1B, lane 2).
As shown in Figure 1C, analysis of cardiac homogenates by Western blot using the same antibody revealed a single immunoreactive band at ∼48 kDa, similar to that reported in aortic tissues [15].
Examination of the overall level of expression by flow cytometry using the same antibody after cell permeabilization indicates that the total protein levels are comparable in these two cell lines (Supplemental Figure S3).
Cells were isolated on ice, after red blood cell lysis, cells were stained for Lin−c-kit+CD41+ using the same antibody clones than mentioned above, except for c-kit which was conjugated to PeCy7 instead of APC.
