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These values are 80 200 times lower than those achievable with ELISA using the same antibodies.
However, in the previous study using the same antibodies as in the present study, Eftekhari et al. reported that 37% of the neurons were immunopositive for CLR and 36% for RAMP1 at a section thickness of 10 12 μm [19].
At ultrastructural level Ts-PCHTP was localized using the same antibodies and protein A-gold.
As a positive control, we analyzed normal human foreskin-derived epidermal melanocyte (NHEM) cells isolated from newborn foreskin using the same antibodies (Figure 3K O).
Indeed, using the same antibodies (F99 and G11) Vascellari et al. [10] found analogous PrPSc deposition patterns in the salivary glands of ARQ/ARQ sheep with scrapie.
These observations were confirmed by immunoblot analysis of cardiac extracts using the same antibodies (Figure 5E) and by RT PCR on cDNA prepared from left ventricle of these mice (Figure S1).
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IHC results for HER2/neu vary with replicate testing using the same antibody clone and when alternate clones are utilized.
Each procedure was repeated a minimum of three times to validate the results and minimize any experimental errors using the same antibody stock.
In line with others using the same antibody [13], we found that circulating CD4+ T cells were effectively deleted.
All combinations of Myc-, V5-, or un-tagged aSyn molecules were stained using the same antibody combination as described above.
Other studies performed in mice have also reported CSPG deposition post trauma using the same antibody [57].
More suggestions(15)
using the indicated antibodies
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using the same plates
using the same tactics
using the polyclonal antibodies
using the same actors
using the monoclonal antibodies
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using the same images
using the appropriate antibodies
using the same weapons
using the following antibodies
using the correct antibodies
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