Sentence examples for using the same amplification from inspiring English sources
We re-amplified aliquots of the DNA by using the same amplification kit and purified the DNA with a standard ethanol precipitation protocol (Note: previous work in our lab had shown that genotype data of pre-amplified and re-amplified genomic DNA from Nasonia embryos are fully consistent).
ex K. D. Koenig were also tested (Van Dijk et al., 2007) using the same amplification conditions.
5′ L1-genome junctions identified in this manner were then validated by PCR followed by standard agarose gel electrophoresis using the same amplification conditions as described above.
Each measurement was normalized to the amount of CrEF1α (Ct) (GenBank accession number BE642078) transcript using the same amplification conditions and the primers 5' CandCCAGTCGGAGCAAAAGT3' and 5'TCCTGTGGGAAGGGTGGAA3'.
Nevertheless, the chosen amplicons were also selected on the basis that all of them may be amplified with similar efficiency using the same amplification program (see Section 2).
Obtained amplicons were also sequenced in both strands using the same amplification primers and analyzed through a BlastX search to confirm their PV origin.
We used the same amplification chemistry on other types of arrays, which harbor probes of the other orientation relative to the transcripts under interrogation (Affymetrix Mouse 430 2.0 arrays), and on this microarray platform we also obtained results that are highly comparable to those from standard processing (data not shown).
We used the same amplification primers for sequencing using Big Dye termination sequencing chemistry (Applied Biosystems).
The clone control samples were amplified using the same PCR amplification protocol as was used for sequencing natural samples.
PCR was carried out for the screening of the cells harboring the recombinant the pHT01-kIspS plasmid using the same primers and amplification conditions as mentioned above.
The amplicon was gel purified and sequenced using the same primers for amplification.
