Furthermore, to improve reproducibility, all steps were automated using the robotic instrument for liquid handling Biomek 3000 Laboratory Automation Workstation, (Beckman Coulter).
In 1998, the scientists, using the robotic arms of the institute's small research submarine, squirted about five pounds of liquid carbon dioxide into a glass beaker more than two miles down.
The biopsy error was larger when using the robotic technique, however not statistically significant.
The robotic liquid handler was used to automate a simple planktonic antimicrobial activity assay for the quaternary ammonium salt CPC, a known antimicrobial that is added to some commercially available mouthwashes and toothpastes.
For the nitrogen limitation assay 96-well-plates were prepared using a robotic liquid handler (FreedomEvo, Tecan) with 150 μl of M9 minimal medium containing different levels of NH4Cl ranging from 0.16 mM to 18.7 mM and 11 mM glucose (without casamino) (each plate contained two different levels of NH4Cl with 48 replicates arranged in a checkerboard format).
All liquid handling and incubation steps were carried out using a robotic liquid handler (Freedom Evo, Tecan, Switzerland) and incubator (StoreX STX44 ICBT, Liconic Instruments, Liechtenstein) system.
Using a robotic liquid handler (FreedomEvo, Tecan), 96-well plates were prepared with 150 μl of M9 minimal medium with sugars as indicated.
Using a robotic liquid handler (Freedom Evo, Tecan), flat bottom 96-well plates (Nunc) were prepared with 150 μl of M9 minimal medium containing 0.2% glucose 0.05% casamino acids, 30 mM cAMP, 50 μg/ml ampicillin and 25 μg/ml.
Samples were prepared using a robotic liquid handling workstation (epMotion 5075, Eppendorf, Germany) and incubated with antibody-coupled beads for 60 min followed by incubation with a detection antibody for 30 min.
