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For validation of the knockdown levels, RNA was isolated using the RNAeasy Kit (Qiagen, Hilden, Germany).
RNA was extracted from the cells using the RNAeasy kit (Qiagen).
Total RNA from 106 sorted CD8+ T cells was isolated using the RNAeasy kit (Qiagen).
Whole RNA was extracted from cells using the RNAeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
For real-time RT-PCR analysis, the total RNA of cells was extracted by using the RNAeasy kit (Qiagen, Valencia, CA).
RNA was isolated from cells using the RNAEasy kit (Qiagen), and reverse transcribed using the first strand cDNA synthesis kit (Invitrogen).
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RNA was isolated from tumor cells preserved in liquid nitrogen or from blood stored at −80°C using the RNAeasy Mini Kit and QIAshredder (QIAGEN, Valencia, CA), or the Ribopure Blood Kit (Ambion, Austin, TX), respectively.
RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) and further purified using the RNAeasy Mini Kit (QIAGEN).
Total RNA from activated wild-type and Ddit3−/− Pmel CD8+ T cells was obtained using the RNAeasy Mini Kit (Qiagen).
The fungal mycelium was collected by filtration on sterile filter paper and 2 to 5 g (wet weight) was used for RNA extraction using the RNAeasy Mini Kit (Qiagen) following the manufacturer's recommendations.
RNA was purified using the RNAeasy mini kit (Qiagen), according to the manufacturer's instructions.
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