Sentence examples for using the reverse transcriptase from inspiring English sources
Reverse transcription was conducted using the reverse transcriptase M-MLV from Promega.
Using the reverse transcriptase polymerase chain reaction (RT-PCR), we cloned two cDNAs with 83% amino acid identity that encode α-amylase-like polypeptides.
cDNA was prepared using the reverse transcriptase kit from Invitrogen.
The influenza virus is traditionally characterized at the molecular level using the reverse transcriptase polymerase chain reaction (RT-PCR).
The RNA preparation was used as the substrate for first strand cDNA synthesis using the reverse transcriptase Superscript III ™ (Invitrogen).
Synthesis of cDNA was performed using the reverse transcriptase Superscript III system (Invitrogen), according to the manufacturer instructions.
Microarray results of selected genes were validated using the reverse transcriptase-multiplex ligation dependent probe amplification (RT-MLPA) method [ 65].
The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR).
Microarray expression of differentially accumulated transcripts identified in the Flynn trial in autumn (Flynn-LW) were validated using the reverse transcriptase-multiplex ligation dependent probe amplification (RT-MLPA) method [ 67].
The expression of mRNA for the epidermal growth factor (EGF) receptor, EGF and transforming growth factor alpha (TGF-alpha) was determined in 76 malignant, six borderline and 15 benign primary ovarian tumours using the reverse transcriptase-polymerase chain reaction and related to clinical and pathological parameters.
To clarify the correlation between French-American-British (FAB) classification and nm23 expression level and to clarify the involvement of nm23-H2 and nm23-H1 in patient survival, we investigated the relative levels of nm23-H1 and -H2 mRNA in 76 AML samples using the reverse transcriptase-polymerase chain reaction.
