Sentence examples for using the reverse primer from inspiring English sources

Randomly assigned clones were sequenced using the reverse primer 5'-CAGATGGGCCCCTGAAGGTA-3'.

The PCR product was purified and directly sequenced using the reverse primer.

All reverse transcription and PCR reactions took place in a single tube using the reverse primer as the reverse transcription primer.

Amplified DNA was gel-purified using the QIAquick Gel Extraction kit (Qiagen, Valencia, CA) and sequenced at Macrogen (Seoul, South Korea) using the reverse primer.

For each sample, we amplified the 16s rRNA gene using the reverse primer 5'-GCCTCCCTCGCGCCATCAGNNNNNNNN CTGCTGCCTYCCGTand' and the forward primer 5'-GCCTTGCCAGCCCGCTCAG AGAGTTTGATCCTGGCTCAG-3'.

To ensure sufficient template for repeat testing, virus sequences were first amplified from 5 µL HIV-1 RNA by reverse transcriptase-polymerase chain reaction (RT-PCR) using the reverse primer RTP-REV2 [5'-CTT CTG TAT GTC ATT GAC AGT CC], and forward primer RTP-F1 [5'-CCT CAG ATC ACT CTT TGG CAA CG], which span from n.t. 1 in protease to n.t.

Downstream breakpoints were determined by aligning the reference sequence with the reverse complements of the sequences generated using the reverse primers.

Amplification of VEGF-C cDNAs was carried out by polymerase chain reaction using the reverse primers: TTG-TTC-GCT-GCC-TGA-CAC-TG and the forward primers: CTC-TCT-CTC-AAG-GCC-CCA-AA.

All the clones with the closest relative being an uncultured bacterium were also sequenced using the reverse primers.

Randomly selected members of each group were sequenced using the reverse primers 1390R and 907R to gain consensus sequences representing each group (almost full length ~1350 bp).

MST3 C-terminal deletion mutants were constructed similarly, but using the reverse primers 5′-CTTATCTAG AAGCTTTCAATCTGTCTCCACATCTGA-3′ [for MST3 Δ314 431)], 5′-CTTATCTAG AAGCTTTCATCCGTTCTCCAGATTCTTGG-3′ [for MST3 Δ341 431)] and 5′-CTTATCTAG AAGCTTTCATTTCAGCTCCGCAAACAGAG-3′ [for MST3 Δ377 431)].