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The rice actin gene was used as the reference gene to normalize the target gene expression, which was calculated using the relative quantization method (2-ΔΔCT).
The ACTIN (GRMZM2G126010) was used as the reference gene to normalize the target gene expression, which was calculated using the relative quantization method (2-ΔΔCT).
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Expression was quantified using the comparative quantization method.
The relative quantization approach through a DeltaDelta CT method was used to analyse the results of real-time PCR experiments.
The relative quantization value of the target, normalized to an endogenous control BMG (housekeeping) gene and relative to a calibrator, was expressed as 2ΔCt, where ΔCt = [Ct of the endogenous control gene (BMG)] - [Ct of the target gene].
The ΔCt method was used to determine relative quantization of miRNA and mRNA expression in samples, and the fold change was determined as 2− ΔΔ Ct.
The computational accuracy is evaluated using the quantization noise power.
The movement of each larva was recorded using the quantization mode.
Differences were calculated according to the ΔΔ Ct relative quantization method using the β-actin gene to calibrate.
The number of quantization levels was 8 for the whole database and the quantization levels were estimated using the Linde-Buzo-Gray (LBG) vector quantization algorithm [22].
The centers for quantization levels were found using the Linde-Buzo-Gray [22] vector quantization algorithm.
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